Science Catalog 2012 Life Cell Signaling DNA Polymerase I Large (Klenow) Fragment Mini Kit Product DNA Polymerase I Large (Klenow) Fragment Mini Kit Available Separately DNA Polymerase I Large (Klenow) Fragment Set of dATP, dCTP, dGTP, dTTP For Laboratory Use. Description: The DNA Polymerase I Large (Klenow) Fragment Mini Kit provides a convenient combination of polymerase and dNTPs. The kit contains 5μmol each of dATP, dGTP, dTTP and dCTP (10mM in water) and DNA Polymerase I Large (Klenow) Fragment, ready for use in a variety of applications. Features: • Convenient: The kit provides DNA Polymerase I Large (Klenow) Fragment and dNTPs conveniently packaged and ready to use in your application. Storage Conditions: Store at –20°C. DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus Product Size Conc. Cat.# Price (Fr) Klenow Fragment, Exonuclease Minus 100 u 5–10 u/µl M2181 89.00 For Laboratory Use. Description: DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and the 3´→5´ exonuclease activities present in intact E. coli DNA Polymerase I. It is used for random primer labeling and in strand displacement amplification. Klenow Fragment, Exonuclease Minus, will leave a single-base 3´ overhang on a significant proportion of DNA fragments during fill-in of 5´-overhangs. Therefore, this enzyme is not recommended for preparation of blunt-ended fragments for ligation. Features: • Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT. • May Be Heat-Inactivated: DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is inactivated by heating at 75°C for 10 minutes. Storage Conditions: Store at –20°C. Protocol DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus Protocol Part# 9PIM218 SP6 RNA Polymerase Product SP6 RNA Polymerase SP6 RNA Polymerase (HC) For Laboratory Use. Size Conc. Cat.# Price (Fr) 1,000 u 10–20 u/µl P1085 227.00 5,000 u 10–20 u/µl P1081 795.00 2,500 u 80 u/µl P4084 426.00 Description: SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only SP6 DNA or DNA cloned downstream from an SP6 promoter can serve as a template for SP6 RNA Polymerase-directed RNA synthesis. Features: • Specific: SP6 RNA Polymerase exhibits extremely high affinity and specificity for SP6 promoter sequences. • Highly Pure: SP6 RNA Polymerase is >90% pure as determined by SDS polyacrylamide gel electrophoresis. Free of detectable levels of contaminating RNase and DNase activity (<1% release). • Flexible: Will incorporate 32P, 33P, 3H and 35S nucleoside triphosphates. • Provided with 5X Reaction Buffer: Provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM spermidine, 50mM NaCl. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Protocol SP6 RNA Polymerase Product Information Part# 9PIP108 Size Cat.# Price (Fr) Size Conc. 150 u U1300 211.00 Cat.# Price (Fr) 150 u 5 u/µl M2201 155.00 10µmol each 100 mM U1330 156.00 T4 DNA Polymerase Product T4 DNA Polymerase For Laboratory Use. Description: T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs. Features: • High Fidelity: T4 DNA Polymerase is the enzyme of choice for applications where misincorporation is a concern. • Flexible: T4 DNA Polymerase may be used in a variety of molecular applications. Active in many Promega 1X restriction enzyme buffers. • May Be Heat-Inactivated: T4 DNA Polymerase is inactivated by heating at 75°C for 10 minutes. • Provided with 10X Reaction Buffer: 250mM Tris-acetate (pH 7.7), 1M potassium acetate, 100mM magnesium acetate and 10mM DTT. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Protocol T4 DNA Polymerase Protocol Part# 9PIM421 Size Conc. Cat.# Price (Fr) 100 u 5–10 u/µl M4211 191.00 500 u 5–10 u/µl M4215 741.00 90 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 93
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