Science Catalog 2012 Life Cell Signaling RNAgents® Denaturing Solution Product RNAgents® Denaturing Solution For Laboratory Use. Size Cat.# Price (Fr) 120 ml Z5651 214.00 Description: RNAgents® Denaturing Solution lyses cells or tissue under conditions that rapidly inhibit ribonucleases, using two potent inhibitors of RNase, guanidine thiocyanate and β-mercaptoethanol. The RNAgents® Denaturing Solution is designed to be used in concert with acidic phenol:chloroform and alcohol (isopropanol) for purification of total RNA. Storage Conditions: Store at 4°C. SV 96 Total RNA Isolation System Product SV 96 Total RNA Isolation System Available Separately RNA Lysis Buffer (RLA) RNA Wash Solution (RWA) Nuclease-Free Water Wizard® SV 96 Binding Plates For Research Use Only. Not for Use in Diagnostic Procedures. Description: The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system also incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation. Protocols are available for Beckman Coulter and PerkinElmer instruments. Features: • Confidence in Results: The product is tested to ensure that purified RNA will perform optimally in RT-PCR. • Unique Design: Novel vacuum manifold eliminates waste handling. Novel plate design prevents cross-contamination during sample processing. • Flexibility: The system is designed for both manual and banden-concurrent.nl/">automated formats. • banden-concurrent.nl/">automation: Validated banden-concurrent.nl/">automated methods available at: www.promega.com/banden-concurrent.nl/">automethods/ • Your Choice of Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store the SV RNA Lysis Buffer with β-Mercaptoethanol (BME) added at 4°C. Store all other components at 22–25°C. Protocol SV 96 Total RNA Isolation System Technical Bulletin Part# TB294 MagaZorb® Total RNA Mini-Prep Kit Product MagaZorb® Total RNA Mini-Prep Kit For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagaZorb® RNA Kit provides an easy, fast and cost-effective technique for isolating PCR-quality total RNA. Using one simple protocol, a high yield of purified total RNA can be isolated from various sources including whole blood (fresh or citrate-, heparin- or EDTA-treated), buffy coat, leukocytes and tissue (fresh or frozen). Features: • Convenient: Contains all needed reagents so that no reagent preparation is required. • Efficient: Eliminates the need for centrifugation, vacuum filtration or column separation, increasing sample throughput and improving reproducibility. • Safe: Does not require organic solvents, eliminating the need for special storage or waste disposal. Storage Conditions: Store at 22–25°C. Protocol MagaZorb® Total RNA Mini-Prep Kit Technical Bulletin Part# TB378 Size Cat.# Price (Fr) 200 preps MB2004 1393.00 Size Cat.# Price (Fr) 1 × 96 each Z3500 503.00 5 × 96 each Z3505 2138.00 50 ml Z3051 72.00 58.8 ml Z3091 71.00 150 ml P1195 211.00 10 pack A2271 1381.00 MagneSil® Total RNA mini-Isolation System Product MagneSil® Total RNA mini-Isolation System For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol enables high-throughput banden-concurrent.nl/">automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well total RNA isolation procedure takes about 30 minutes to complete using a liquid-handling workstation. Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR. Features: • Improve Productivity: Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler. • Improve Real-Time PCR Performance: Elution volumes as low as 15μl provide concentrated RNA without the need for time-consuming vacuum concentration. • Gain Confidence in Results: DNase I treatment is included to remove genomic DNA contamination. • Achieve Convenience: Robotic protocols require no user intervention once you start the banden-concurrent.nl/">automated robotic method. • banden-concurrent.nl/">automate This Assay: Validated banden-concurrent.nl/">automated methods are available at: www.promega.com/banden-concurrent.nl/">automethods/ Storage Conditions: Store at 22–25°C. Protocol MagneSil® Total RNA mini-Isolation System Technical Bulletin Part# TB328 Size Cat.# Price (Fr) 4 plate Z3351 1666.00 126 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 129
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