Cell Signaling SV Total RNA Isolation System Product SV Total RNA Isolation System Available Separately Red Blood Cell Lysis Solution (CLB) RNA Lysis Buffer (RLA) 200 ml Z3141 82.00 50 ml Z3051 72.00 Z3101, Z3141, Z3100, Z3105 For Laboratory Use. Z3051, Z3380, Z3382, Z3384 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour. Using this membrane-based purification system, up to 60mg of tissue can be processed per purification, depending on tissue type. The system incorporates a DNase treatment step directly on the minicolumn membrane. This step substantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carryover in the final RNA preparation. Features: • Safety and Efficiency: Rapid isolation of high yields of total RNA without the use of hazardous compounds like phenol. • Flexibility: Single system for isolation directly from blood, cells or tissue. Two methods available for purification: microcentrifugation (spin) or vacuum. • Confidence: Purified RNA suitable for all routine molecular biology applications, including RT-PCR and Northern blotting. Storage Conditions: Store at 22–25°C. Protocol SV Total RNA Isolation System Protocol β-actin M 5µl bp 1,500 – 1,000 – 700 – 400 – APC 5µl 10µl 15µl Part# TM048 Size Cat.# Price (Fr) 10 preps Z3101 110.00 50 preps Z3100 330.00 250 preps Z3105 1403.00 Samples Mouse Tissues Liver Kidney Spleen Brain Muscle Rat Tissues Pancreas Heart Lung Bacteria E. coli Plant Tomato Leaf Cell Line RAW264.7 N/A = Not applicable 5 ⋅ 106 cells 51 N/A 2.1 9485LA 30mg 4.6 0.15 2.0 Average Yields of Total RNA Isolated Using SV Total RNA Isolation System. Maximum Avg. Yield Avg. Yield Amt. to per Prep per mg Process (µg) 30mg 20mg 15mg 60mg 30mg 30mg 60mg 60mg 131 44 79 39 22 100 16 36 1 ⋅ 109 cells 36 Yeast S. cerevisiae 4 ⋅ 107 cells 19 4.4 2.2 5.3 0.65 0.73 3.5 0.27 0.6 N/A N/A Tissue (µg) A260/A280 1.9 1.9 1.9 2.1 2.1 1.9 2.1 2.1 2.0 2.1 5 RNA was isolated from 1ml of human blood using the SV Total RNA Isolation System. RT-PCR was performed using the indicated volumes of eluted RNA and primers complementary to human β-actin or human adenomatous polyposis coli (APC) gene with the Access RT-PCR System (Cat.# A1250). Lane M = 100bp DNA Ladder (Cat.# G2101). For complete and up-to-date product information visit: www.promega.com/catalog 125 DNA and RNA Purification Pagina 128
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