Ajoutez-Agitez-Mesurez ! CellTiter-Glo®3D est un test simple d’utilisation. Ajoutez simplement le réactif aux cellules dans leur milieu de culture et mesurez la viabilité cellulaire des micro-tissus en 3D après 30 minutes. Ce test luminescent peut être adapté à un criblage haut débit et est compatible avec de nombreuses méthodes de culture en 3D. Il offre également une sensibilité plus élevée que les tests de viabilité cellulaire colorimétriques ou fluorimétriques. A. HCT116 Human Cancer Cell Microtissues 1,000 10,000 1,000 100 10 10 1 CellTiter-Glo® 3D alamarBlue® MTT 1 CellTiter-Glo® 3D alamarBlue® MTT Figure 1. celltiter-Glo® 3D assay provides better signal-to-background ratios than colorimetric or fluorometric assays. Panel A. HCT116 colon cancer cells seeded into an InSphero GravityPLUS™ 96-well hanging-drop platform and grown to generate ~340μm spheroids. Panel B. InSphero Insight™ human liver microtissues (~250μm). All microtissues were assayed using the CellTiter-Glo® 3D, alamarBlue® and MTT assays according to manufacturer’s protocols. Total assay times for the CellTiter-Glo® 3D, alamarBlue® and MTT assays were 30 minutes, 3 hours and 8 hours, respectively. A. Hanging Drop Method 120 100 80 60 40 20 0 –10 –9 –8 –7 Log [Compound], M C. Matrigel® 120 100 80 60 40 20 0 Figure 2. celltiter-Glo® 3D assay is compatible with a variety of 3D culture methods. HCT116 colon cancer cells were seeded as follows: 400 cells in hanging-drop (Panel A); 1,000 cells in ultra-low attachment (ULA) or Matrigel® (Panels B and C). Microtissues were grown for 4 days, treated with compounds for 48 hours, then assayed with the CellTiter-Glo® 3D Reagent. Luminescence was recorded at 30 minutes. –10 –9 –8 –7 Log [Compound], M enews | Juillet 2015 –6 –5 –4 –6 –5 panobinostat terfenidine trichostatin A B. ULA –4 120 100 80 60 40 20 0 100 B. Human Liver Microtissues –10 –9 –8 –7 Log [Compound], M –6 –5 –4 Percent Relative Activity Percent Relative Activity Signal-to-Background Ratio Signal-to-Background Ratio 12386MB Percent Relative Activity 12331MC Pagina 4

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