Cell Signaling Conventional DNA Markers Product Lambda DNA/HindIII Markers Lambda DNA/EcoRI Markers Lambda DNA/EcoRI + HindIII Markers ΦX174 DNA/HaeIII Markers ΦX174 DNA/HinfI Markers pGEM® DNA Markers For Laboratory Use. Description: The Conventional DNA Digest Markers are created by digesting either λ DNA, ΦX174 replicative form DNA, or plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. Size Conc. Cat.# Price (Fr) 100 µg 0.5 µg/µl G1711 88.00 100 µg 0.5 µg/µl G1721 88.00 100 µg 0.5 µg/µl G1731 88.00 50 µg 1 µg/µl G1761 244.00 50 µg 1 µg/µl G1751 244.00 50 µg 1 µg/µl G1741 210.00 λ DNA/HindIII Markers: Eight ethanol-precipitated DNA fragments ranging in size from 125bp to 23,130bp. λ DNA/EcoRI Markers: Six ethanol-precipitated DNA fragments ranging in size from 3,530bp to 21,226bp. λ DNA/EcoRI + HindIII Markers: Thirteen ethanol-precipitated DNA fragments ranging in size from 125bp to 21,226bp. ΦX174 DNA/HaeIII Markers: Eleven phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 72bp to 1,353bp. ΦX174 DNA/HinfI Markers: Twenty ethanol-precipitated DNA fragments ranging in size from 24bp to 726bp. pGEM® DNA Markers: Fifteen phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM®-3 Vector DNA with HinfI, RsaI and AvaII later combined to form the markers. Recommended Loading: Load 1μl/lane. Storage Conditions: Store at –20°C. 4 bp – 1,353 bp bp – 23,130 – 9,416 – 6,557 – 4,361 – 2,322 – 2,027 [564, 125] bp – 21,226 – 21,226 – 7,421 – 4,878 – 3,530 5,804 5,643 – 4,268 – 3,530 5,148 4,973 – 2,027 ––––––– 1,904 – 1,584 – 1,375 – 947 – 831 [564, 125] 0.7% agarose Lambda DNA/ HindIII Markers Cat.# G1711 Load 1µl/lane. 0.7% agarose Lambda DNA/ EcoRI Markers Cat.# G1721 Load 1µl/lane. 0.7% agarose Lambda DNA/EcoRI + HindIII Markers Cat.# G1731 Load 1µl/lane. – 118 [72] 8% acrylamide φX174 DNA/ HaeIII Markers Cat.# G1761 Load 1µl/lane. ––––– 271 – 310 – 281 – 234 – 194 – 603 bp bp – 726 – 553 – 500 – 311 ––––– 140 – 249 – 200 – 151 – 118 – 100 – 82 – 66 – 24 48 42 40 – 2,645 ––––– 713 427 417 413 – 1,605 – 1,198 – 676 – 517 – 460 – 396 – 350 – 222 – 179 – 126 – 75 – 65 – 51 [36] 8% acrylamide φX174 DNA/ HinfI Markers Cat.# G1751 Load 1µl/lane. 8% acrylamide pGEM® Cat.# G1741 Load 1µl/lane. Markers DNA – 1,078 – 872 For complete and up-to-date product information visit: www.promega.com/catalog 75 Cloning and DNA Markers 1258TB09_5A 1258TA09_5A 1258TC09_5A 1238TA09_5A 0265TA05_2A 0266TA05_1A 8655TA Pagina 78
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Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home