Science Catalog 2012 Life Cell Signaling A. Anti-ACTIVE® MAPK pAb NGF-Treated Product Untreated Anti-ERK 1/2 pAb, Rabbit Anti-ACTIVE® MAPK pAb kDa 98 – 64 – 50 – B. Anti-ACTIVE® JNK pAb Sorbitol-Treated Untreated 36 – 1 2 1 2 Detection of the specifically phosphorylated form of MAPK in NGFtreated PC12 cell extracts. Anti-ACTIVE® MAPK pAb (Cat.# V8031) and Anti-ERK 1/2 (“pan ERK 1/2”) pAb (Cat.# V1141) detection of ERK 1/2 in untreated (lanes 1) or NGF-treated (lanes 2) PC12 cell extracts (2µg). Product Donkey Anti-Rabbit IgG (H+L), HRP C. Anti-ACTIVE® p38 pAb Sorbitol-Treated Untreated Size Cat.# Price (Fr) 60 µl V7951 197.00 Protein Kinase Inhibitors and Activators Protein Kinase Inhibitors and Activators Product MEK Inhibitor U0126 Size Cat.# Price (Fr) 5 mg V1121 422.00 Detection of activated MAPK, JNK and p38 in PC12 cells by immunocytochemistry. PC12 cells were grown to 80% confluence in RPMI 1640 medium supplemented with 25mM HEPES, 300mg/L l-glutamine, 10% horse serum, 5% fetal bovine serum and 0.5mM EGTA. Cells were either untreated or treated with 200ng/ml NGF or 1M sorbitol as indicated. ICC was performed as described in Promega Technical Bulletin #TB262. Anti-ACTIVE® antibodies were used at the following dilutions: Panel A. MAPK, 1:500; Panel B. JNK, 1:1,000; Panel C. p38, 1:500. Protocols developed and performed at Promega. cAMP-Dependent Protein Kinase Peptide Inhibitor 1 mg V5681 297.00 Myristoylated Protein Kinase C Peptide Inhibitor InCELLect™ AKAP St-Ht31 Inhibitor Peptide InCELLect™ St-Ht31P Control Peptide Olomoucine (cdc2 Protein Kinase Inhibitor) PD 98059 SB 203580 LY 294002 PMA 4α-PMA cGMP, 1mM cAMP, 1mM 1 mg V5691 140.00 150 µl V8211 576.00 150 µl V8221 576.00 0.5 mg V2372 108.00 10 mg V2373 540.00 5 mg V1191 196.00 1 mg V1161 248.00 5 mg V1201 275.00 5 mg V1171 270.00 1 mg V1181 178.00 500 µl V6411 68.00 500 µl V6421 68.00 Size Cat.# Price (Fr) 40 µl V1141 388.00 Anti-ERK 1/2 pAb 68 For complete and up-to-date product information visit: www.promega.com/catalog 2870TA02_0A 2582TB03_9A Pagina 71
Pagina 73Voor club bladen, online tijdschriften en club bladen zie het Online Touch CMS beheersysteem systeem. Met de mogelijkheid voor een webshop in uw sportbladen.
Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home