Science Catalog 2012 Life Cell Signaling Caspase-Glo® 6 Assay Systems Product Caspase-Glo® 6 Assay For Laboratory Use. Description: The homogenous Caspase-Glo® 6 Assay measures caspase-6 acitivity. Caspase-6 is a member of the cysteine aspartic acid-specific protease family and has a key effector role in the cleavage of specific target proteins during apoptosis. The Caspase-Glo® 6 Assay provides a luminogenic substrate, Z-VEID-aminoluciferin, in a buffer optimized for caspase-6 and luciferase activity. The addition of a single Caspase-Glo® 6 Reagent results in cleavage of the substrate, releasing aminoluciferin, and generating a glow-type luminescent signal in the presence of Ultra-Glo™ Recombinant Luciferase. The luminescent signal is proportional to the amount of caspase-6 activity. The Caspase-Glo® 6 Assay is designed for use with purified enzyme preparations in multiwell plate formats, making it ideal for banden-concurrent.nl/">automated high-throughput screening for caspase-6 activity and inhibitors of caspase-6 activity. Features: • Simplified Method: The protocol makes the assay easy to banden-concurrent.nl/">automate. • Greater Sensitivity: The assay is more sensitive than fluorescence-based caspase-6 assays and provides excellent signal-to-noise ratios. The assay is linear over 3 logs of caspase-6 concentration and can detect 0.002U/ml. • Increased Accuracy: The superior sensitivity over fluorescence-based caspase assays allows inhibitor studies at concentrations below the Km. • Faster Results: The maximum signal and sensitivity of the assay is reached in as little as 30 minutes after reagent addition. • High-Quality Assay: The assay delivers an excellent Z´-factor value of 0.86 when using 0.1U/ml of caspase-6 for assays in 384-well plates. • Batch Processing Capability: Stable, continous signall allows plates to be read over an extended period of time. Storage Conditions: Store at –20°C. Protocol Caspase-Glo® 6 Assay Technical Bulletin Part# TB366 For Laboratory Use. Description: The homogeneous Caspase-Glo® 8 Assay measures caspase-8 activity using a proluminogenic caspase-8 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 8 Reagent in an “add-mix-measure” format results in cell lysis, followed by caspase cleavage of the substrate and generation of a “glowtype” luminescent signal. The signal generated is proportional to the amount of caspase activity present. The Caspase-Glo® Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates the stable “glow-type” luminescent signal and improves performance across a wide range of assay conditions. The system now includes a separate vial of MG-132 protease inhibitor, which may be used to reduce background, improving the performance of the Caspase-Glo® 8 Assay in cell-based applications. Features: • Simplify Apoptosis or Caspase Detection: The homogeneous “addmix-read” protocol makes the assay easy to banden-concurrent.nl/">automate; simply add an equal volume of reagent to sample volume. • Use Less Enzyme: Excellent signal-to-noise ratios and superior sensitivity allow assays to be performed in 96- or 384-well formats. • Decrease Assay Time: No sample preparation or manipulation required, and no extended incubation times are necessary as with fluorescent-based assays. Maximum sensitivity is achieved in as little as 0.5–1 hour. • Rely on a Performance-Tested Assay: The assay delivers excellent Z´ factor values in cell and purified enzyme models. • Get More Information: Multiplex with other cell-based assays from Promega. • Experience Improved Caspase-8 Selectivity: Caspase-Glo® 8 Assay uses a luminogenic substrate containing the LETD sequence, which has been shown to be selective for caspase-8. The assay includes an optional proteasome inhibitor (MG-132), which when added to the Caspase-Glo® 8 Reagent significantly reduces nonspecific background in cell-based assays. • banden-concurrent.nl/">automate This Assay: Validated banden-concurrent.nl/">automated methods available at: www.promega.com/banden-concurrent.nl/">automethods/ • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C protected from light. Protocol Caspase-Glo® 8 Assay Technical Bulletin Part# TB332 Size Cat.# Price (Fr) 10 ml G0970 809.00 50 ml G0971 2832.00 Caspase-Glo® 8 Assay Systems Product Caspase-Glo® 8 Assay Size Cat.# Price (Fr) 2.5 ml G8200 231.00 10 ml G8201 809.00 100 ml G8202 4045.00 28 For complete and up-to-date product information visit: www.promega.com/catalog Pagina 31
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