Science Catalog 2012 Life Cell Signaling Product ChipShot™ Labeling and Clean-Up Systems Size Cat.# Price (Fr) ChipShot™ Indirect Labeling and Clean-Up System ChipShot™ Direct Labeling and Clean-Up System 25 reactions Z4000 Pls. Enq. 25 reactions Z4100 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: The ChipShot™ Labeling and Clean-Up Systems enable researchers to generate consistent, reproducible and sensitive microarray data. The ChipShot™ Systems make it easy for individual researchers to perform RNA and microarray manipulation, regardless of their experience. The protocols are simple, time-tested and easy-to-follow. The ChipShot™ Direct System provides quality reagents and enzymes for generating fluorescently labeled cDNA via direct incorporation of fluorescently modified nucleotides. Labeled cDNA then can be hybridized to a printed microarray. The ChipShot™ Indirect System provides quality reagents and enzymes for generating fluorescently labeled cDNA using a two-step labeling process that includes synthesis of aminoallyl-modified cDNA, followed by conjugation to the CyDye™ NHS ester. Labeled cDNA then can be hybridized to a printed microarray. Note: Customers must supply CyDye™ nucleotides or NHS esters. Features: ChipShot™ Systems • Conserve Sample: Optimized protocols for 5μg of total RNA or 1.5μg of poly(A)+ RNA per labeling reaction. • Rely On Quality Products: Quality-controlled reagents are always available from Promega. • Simplify Your Process: Easy-to-follow protocols. • Increase Productivity: No hybridization reagent preparation and testing. • Count On Technical Support: Outstanding technical support from Promega for the researcher. ChipShot™ Direct System • Increase Productivity: RNA to purified, labeled cDNA in approximately 3 hours. • Get Reliable Results: Separate protocols for CyDye™3 and CyDye™5 labeling, so dye swap experiments are not necessary. ChipShot™ Indirect System • Achieve Higher Yields: Efficient incorporation of aminoallyl dNTP by reverse transcriptase yields more cDNA for labeling. • Get Unbiased Dye Incorporation: CyDye™3- and CyDye™5-label cDNA at a comparable rate. cDNA yield achieved using the ChipShot™ Membrane Clean-Up System compared to competing systems. Storage Conditions: Store the ChipShot™ Direct Labeling System at –20°C, except for the RNase A, which should be stored at room temperature, and the Total RNA Positive Control, which should be stored at –70°C. Store the ChipShot™ Indirect Labeling System at –20°C, except for the RNase Solution, which should be stored at room temperature, and the Total RNA Positive Control, which should be stored at –70°C. Protocol ChipShot™ Indirect Labeling and Clean-Up System Technical Manual Part# TM287 ChipShot™ Direct Labeling and Clean-Up System Technical Manual TM286 1,000 1,200 1,400 1,600 1,800 2,000 200 400 600 800 0 290 For complete and up-to-date product information visit: www.promega.com/catalog cDNA Yield (ng) 5356MB Competitor A Competitor B ChipShot™ Membrane Clean-Up System Paramagnetic Particles Pagina 293

Pagina 295

Interactieve digitale archief, deze weekblad of mailing is levensecht online geplaatst met Online Touch en bied het online bladerbaar maken van web spaarprogramma.

Promega Switzerland - Life Sciences Catalog 2012 Lees publicatie 100Home