Cell Signaling Apoptosis       VIABLE T0                            NECROSIS               APOPTOSIS T2                                REDUCED VIABILITY T1 2  Assay Type CellTiter-Glo® Assay CellTiter-Fluor™ Assay CellTiter-Blue® Assay CellTiter 96® AQueous One Solution Assay MultiTox-Glo Assay MultiTox-Fluor Assay CytoTox-Glo™ Assay CytoTox-Fluor™ Assay CytoTox-ONE™ Assay Caspase-Glo® 3/7 Assay Parameter/Biomarker Measured Viable cell ATP Live-cell protease Resazurin reduction by NADH MTS reduction by NADH Viability and cytotoxicity by live- and dead-cell proteases Viability and cytotoxicity by live- and dead-cell proteases Dead-cell protease release Dead-cell protease release LDH release Caspase-3/7 activity Apo-ONE® Caspase 3/7 Assay Caspase-3/7 activity Caspase-Glo® 8 Assay Caspase-Glo® 9 Assay GSH-Glo™ Assay GSH/GSSG-Glo™ Assay Caspase-8 activity Caspase-9 activity Mitochondrial ToxGlo™ Assay ATP Dead cell protease GSH GSH/GSSG Time to Results Sensitivity (*384 well) Plate Format Instrument 10 minutes 10 viable cells* 96/384/1536 Luminometer/CCD 0.5–3 hours 40 viable cells 96/384/1536 1–4 hours 50 cells* 1–4 hours 200 cells* 0.5 hour 40 viable cells, 10 dead cells 0.5–3 hours 40 live cells, 10 dead cells 15 minutes 10 dead cells 0.5–3 hours 10 dead cells 10 minutes 200 cells* 0.5 hour 20 cells* 1–18 hours 200 cells* 0.5 hour 0.5 hour 1 hour 30 minutes 1hour ~1000 cells ~1500 cells 96/384/1536 96/384 96/384/1536 96/384/1536 Fluorometer AFC 400nmEx/505nmEm Fluorometer, Resorufi n 560nmEx/590nmEm Spectrophotometer Abs 490nm AFC 400nmEx/505nmEm Luminometer Fluorometer Fluorometer AFC 400nmEx/505nmEm R110 485nmEx/520nmEm 96/384/1536 Luminometer 96/384 96/384 Fluorometer R110 485nmEx/520nmEm Fluorometer, Resorufi n 560nmEx/590nmEm 96/384/1536 Luminometer 96/384/1536 96 96 10 viable cells 96/394 10 dead cells 96\384 96\384 Fluorometer R110 499nmEx/521nmEm Luminometer Luminometer Luminometer Luminometer Luminometer 9481LA For complete and up-to-date product information visit: www.promega.com/catalog 25 Cell Health Assays  Pagina 28

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