Cell Signaling EnduRen™ Live Cell Substrate Product EnduRen™ Live Cell Substrate For Research Use Only. Not for Use in Diagnostic Procedures. Description: EnduRen™ Live Cell Substrate provides new capabilities in performing luminescent reporter assays by enabling live cell kinetic measurements, streamlining assay development and multiplexing with other lytic assays. EnduRen™ Live Cell Substrate provides the ability to measure Renilla luciferase luminescence for at least 24 hours after substrate addition, with up to tenfold higher signal-to-background ratios than wildtype coelenterazines. EnduRen™ Live Cell Substrate is a uniquely engineered coelenterazine with protected oxidation sites, which minimizes substrate degradation and banden-concurrent.nl/">autoluminescence (background) in cell culture, while it extends the luminescent signal to accommodate microplates without the need for banden-concurrent.nl/">auto-injectors. The result is that EnduRen™ Live Cell Substrate overcomes the key limitations of wildtype coelenterazines by providing an banden-concurrent.nl/">automation-friendly, highly sensitive substrate for Renilla luciferase-based gene reporter and BRET applications. Features: • Live Cell Assay: Generate kinetic profiles for reporter gene, BRET and RNAi applications. • Kinetic Reporter Gene Analysis: Conserve test compounds as you create response profiles in real time to generate more data-rich results. • Streamlined Assay Development and Screening: Rapidly obtain optimal assay parameters through repeat measurements using only a single cell population. Increase your sample throughput using microplates without time-consuming per-sample reagent injection steps. • Designed for Multiplexing: Perform more dynamic experiments using the same sample set by pairing with any lytic assay. • High Signal-to-Background Ratios: Reliably quantitate low levels of expression for reporter gene detection and BRET. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store at –20°C. Protocol EnduRen™ Live Cell Substrate Technical Manual Part# TM244 Size Cat.# Price (Fr) 0.34 mg E6481 224.00 3.4 mg E6482 1680.00 34 mg E6485 Pls. Enq. Isoproterenol (6µM) 10 12 0 2 4 6 8 Forskolin (10µM) 15 06 12 18 24 Time (hours) Real-Time live-cell reporter assay using the EnduRen™ Substrate. Luminescence was monitored from HEK 293 cells for >24 hours, permitting measurement of the effects of sequential treatment of the cells with isoproterenol and forskolin. 30 36 For complete and up-to-date product information visit: www.promega.com/catalog 267 Reporter Assays and Transfection Fold Induction 4273MA08_3A Pagina 270
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