Science Catalog 2012 Life Cell Signaling HaloLink™ Magnetic Beads Product HaloLink™ Magnetic Beads For Research Use Only. Not for Use in Diagnostic Procedures. Description: HaloLink™ Magnetic Beads provide a rapid and reliable method to covalently capture and immobilize HaloTag® fusion proteins to a paramagnetic particle. Immobilization through the HaloTag® Ligand provides for consistent, surface-directed orientation of the fusion protein, thus providing more reliable results in applications such as protein:protein interaction studies with the fusion protein. The result is a paramagnetic bead with very high binding capacity for HaloTag® fusion proteins and very low nonspecific protein binding. The paramagnetic feature allows for streamlined assay development and facilitates applications with banden-concurrent.nl/">automated assays developed on robotic platforms. A variety of vectors for the expression of HaloTag® fusion proteins in bacterial, mammalian or cell-free systems are available. Please see the HaloTag® Technology Products page for more information. HaloTag® Fusion Protein (bait) Size Cat.# Price (Fr) 40 reactions G9311 155.00 Features: • Magnetic-Based: Easy and quick immobilization of HaloTag® fusion proteins with minimal loss of sample material. • Covalent Bond: Allows stringent washing of immobilized HaloTag® fusion protein, removing nonspecific proteins and enhancing overall data. • Multiple Applications: Immobilized HaloTag® fusion proteins can be used for protein interaction analysis, enzyme assays and purification of fusion proteins containing a protease cleavage site. Storage Conditions: Store at 4°C. Protocol HaloLink™ Magnetic Beads Technical Manual Part# TM291 Add HaloLink™ Magnetic Beads B (protein Bind immobilization) B Wash Remove nonspecific proteins B Potential downstream applications Detection of Protein: Protein Interactions HaloTag® Fusion Protein B Overview of the HaloLink™ Magnetic Beads immobilization protocol. binding partner Detection of Enzymatic Activity A substrate B B product B Purification of Nontagged Protein Factor Xa cleavage 250 For complete and up-to-date product information visit: www.promega.com/catalog 6630MA Pagina 253
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