Cell Signaling StemElite™ Differentiation-Associated Transcripts Product StemElite™ FOXA2/GAPDH Primer Pair (20X) StemElite™ SOX17/GAPDH Primer Pair (20X) StemElite™ GATA6/GAPDH Primer Pair (20X) For Research Use Only. Not for Use in Diagnostic Procedures. Description: Pluripotential stem cells can give rise to differentiated cells and tissues for all three embryonic germ layers. FOXA2, SOX17 and GATA6 are nonspecific differentiation markers. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplexed amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (GAPDH). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring all of the transcripts in the same well. Storage Conditions: Store at –20°C. StemElite™ Mouse Pluripotent Transcripts Product StemElite™ Mus-Nanog/Actb Primer Pair (20X) StemElite™ Mus-Sox2/Actb Primer Pair (20X) StemElite™ Mus-Pou5f1/Actb Primer Pair (20X) StemElite™ Mus-Lin28/Actb Primer Pair (20X) StemElite™ Mus-Klf4/Actb Primer Pair (20X) StemElite™ Mus-Myc/Actb Primer Pair (20X) For Research Use Only. Not for Use in Diagnostic Procedures. Size Cat.# Price (Fr) 100 µl B1371 133.00 100 µl B1381 133.00 100 µl B1391 133.00 100 µl B1401 133.00 100 µl B1411 133.00 100 µl B1421 133.00 Description: Mus-Nanog, Mus-Sox2, Mus-Pou5f1, Mus-Lin28, Mus-Klf4 and Mus-Myc are functionally associated with maintenance of the undifferentiated mouse embryonic stem cell. Features: • Achieve sensitive real-time detection and relative quantification of RNA expression levels for monitoring stem cell differentiation in a multiplexed amplification. • Quantitatively amplify a two-color duplex. • Amplify in a single tube the transcript of interest and reference transcript (Actb). • Reduce the number of reactions required to measure multiple transcripts. • Improve experimental data quality by measuring all of the transcripts in the same well. Storage Conditions: Store at –20°C. Size Cat.# Price (Fr) 100 µl B1341 133.00 100 µl B1351 133.00 100 µl B1361 133.00 RT-PCR GoScript™ Reverse Transcription System Product GoScript™ Reverse Transcription System Available Separately GoScript™ Reverse Transcriptase For Laboratory Use. Description: The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents designed for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® qPCR systems for performing RT-qPCR. Features: • Ultra-Active: Save money on every reaction. • Sensitive: Detect rare transcripts. • Processive: Transcribe long messages. • Resilient: Synthesize cDNA in the presence of strong inhibitors. • RT Replaces Superscript® I, II, III and other RTs. Storage Conditions: Store at –20°C. Protocol GoScript™ Reverse Transcription System Technical Manual Part# TM316 100 reactions A5003 362.00 500 reactions A5004 1539.00 Size Cat.# Price (Fr) 50 reactions A5000 461.00 100 reactions A5001 875.00 12 For complete and up-to-date product information visit: www.promega.com/catalog 215 PCR Pagina 218
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