A,
B,
Panel A. Human total RNA was reverse transcribed using oligo(dT) according to the manufacturer’s instructions with the exception that the GoScript™ Reverse Transcription reaction was carried out at 50˚C, as recommended for SuperScript® III. cDNA was analyzed by amplification of b-2 macroglobulin (B2M, control) or GC-rich Nocturnin using GoTaq® Green Master Mix (Cat.0x M7122). Panel B. Human total RNA was again reverse transcribed but at each enzyme’s recommended reaction temperature. cDNA was analyzed by real-time PCR for both transcripts using GoTaq® qPCR Master Mix (Cat.0x A6001). Data are the average of three independent experiments.
• erformance when detecting trace P transcripts by real-time PCR using long RNA templates • ensitivity to ethanol, a common S contaminant in RNA preparations • bility to transcribe a challenging A GC-rich mRNA In terms of sensitivity of transcript detection, transcript length and sensitivity to ethanol contamination, the new GoScript™ Reverse Transcriptase performed as well as or better than Invitrogen SuperScript® II and SuperScript® III and Qiagen Omniscript® and Sensiscript® reverse transcriptases. In a comparison of GoScript™
and SuperScript® III using a GC-rich transcript and an elevated reaction temperature (50°C), GoScript™ Reverse Transcriptase outperformed SuperScript® III in the amount of cDNA produced as determined by quantitative PCR. GoScript™ Reverse Transcriptase is more efficient using templates with complex secondary structure. Read more about how GoScript compares in: Lining Up The Scripts: A Comparison of Reverse Transcriptases.
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Select the most reliable cell-free expression system for your specific research needs from the largest product portfolio commercially available. Choose between eukaryotic- and prokaryotic-based expression systems for either DNA or RNA templates. Promega TNT® Systems offer convenient, single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. Using the TNT® Quick Master Mix, a one-hour reaction produces sufficient quantities of protein that can be used directly for a variety of applications. These include protein:protein interactions, protein:nucleic acid interactions and protein modification. For maximum yield, our S30 T7 High-Yield Protein Expression System is designed to express up to
500μl/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. This highly optimized system results in greater stability and enhanced expression of target proteins.
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