Kinase Activity Assays with ADP-Glo™ Kinase Assay Kinase reaction ATP PPP P Substrate OR Pi Step 2. Add Kinase Detection Reagent. ATPase reaction ATP PPP Your Reaction ADP P P Reaction Products ADP P P Convert ATP Newly synthesized ATP. ADP-Glo™ Assay PP P Promega Kinase Enzyme Systems provide the kinase enzyme, substrate, buffer, and ADP-Glo™ reagents in an integrated, easy-to-use protocol for quantifying kinase activity. Over 170 kinases, including lipid kinases, are available. Luciferase/Luciferin Detect Light Step 1. Add ADP-Glo™ Reagent. Substrate ATP PP P Remaining ATP. ATP PP P ATP remaining after your reaction. Deplete ATPr PP P Request a Sample enews | July 2013 Pagina 4

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