erse Transcriptase to specific applications than others. The downstream application, length of the target ctivity are all important considerations when choosing the right reverse transcriptase. ences between different enzyme choices. M-MLV RNase H– Reverse Transcriptase High-performance enzyme for routine cDNA synthesis and RT-PCR. Lack of RNase H activity makes this the enzyme of choice for reverse transcribing long RNAs. 42°C AMV Reverse Transcriptase Recommended for reverse transcription of RNAs >5kb and primer extension, particularly if the template RNA has strong secondary structure. 42°C Access and AccessQuick™ RT-PCR Systems Ordering Information Equivalent toSuperScript® II Read the Article Ordering Information AMV enews | September 2013 Pagina 12
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